Virginia joined Florida, Wichita State and Arizona as a No. 1 seed Sunday when the selection committee announced the pairings for the NCAA tournament.
The Cavaliers, widely considered a 2 or 3 seed in many mock-ups of the bracket, instead vaulted to the top of the East region. They won the ACC regular-season and tournament titles.
March Madness begins Tuesday with a pair of "First Four" games in Dayton, Ohio.
The Gators bring a 26-game winning streak into the tournament. They are seeded first in the South and are the No. 1 seed overall.
Wichita State, the top seed in the Midwest, comes in at 34-0 - the first team to enter the tournament undefeated since UNLV in 1991.
The Final Four starts April 5 in Arlington, Texas.